Changwen Jin
Ph.D, Professor
email: changwen@pku.edu.cn(link sends e-mail)
fax: +86-10-62753790
office phone: +86-10-62756004
Research Group URL
Research Experience
Research Areas
Our research mainly focuses on studies of the structures, dynamics and biological functions of proteins, especially enzymes. In particular, we are interested in the correlation between protein dynamics (structural fluctuations over time) and their functional mechanisms. We employ Nuclear Magnetic Resonance (NMR) technique to determine the solution structures and probe the dynamic properties of bio-macromolecules. We also use biochemical methods to characterize the activity and functions of proteins both in vitro and in vivo. Our main goal is to elucidate the structural and dynamic basis of protein functions.
Protein structures and dynamics
To provide structural basis for their biological functions, we use NMR technique to determine the solution structures of proteins and protein complexes. However, protein functions essentially depend on their motions, and static structures alone cannot fully explain their functions. NMR is a powerful tool to probe the motional properties of proteins. Therefore, we use relaxation phenomena, as well as newly developed NMR methods such as PRE and RDC to investigate protein dynamics and their correlation to functions.
Protein interactions
Protein functions through interactions with other molecules (proteins, ligands, or nucleic acids). We use NMR methods to characterize protein interactions. In particular, we are interested in studying (1) interactions between enzymes and substrates during catalysis, (2) interactions between receptors and ligands in signal transduction pathways, (3) protein-protein interactions in the regulation of proteasome.
Membrane proteins
Membrane proteins play essential roles in cells. Recent advances in biochemical and NMR techniques have made it possible to use solution NMR method to study the structures and dynamics of membrane proteins. We are interested in studying the structures of protein cross-membrane translocation machineries, and to elucidate the molecular mechanism or the translocation processes.
Representative Publications
53. Guan L1, He P1, Yang F1, Zhang Y1, Hu Y1, Ding J1, Hua Y, Zhang Y, Ye Q, Hu J, Wang T*, Jin C*, Kong D*. Sap1 is a replication initiation factor essential for the assembly of pre-replicative complex in the fission yeast Schizosaccharomyces pombe. J. Biol. Chem. 10.1074/ jbc. M166. 767806. [Epub ahead of print] ( 1 equal contributions )
52. Yu C, Niu X, Jin F, Liu Z, Jin C, Lai L*. Structure-based Inhibitor Design for the Intrinsically Disordered Protein c-Myc. Sci Rep. 6, 22298, 2016.
51. Wei H, Wang L, Ren X, Yu W, Lin J, Jin C, Xia B*. Structural and functional characterization of tumor suppressors TIG3 and H-REV107.FEBS Lett. 589 (11), 1179-86, 2015
50. Jienv Ding, Chengfeng Yang, Xiaogang Niu, Yunfei Hu* and Changwen Jin*. HdeB chaperone activity is coupled to its intrinsic dynamic properties. Sci Rep 5, 16856, 2015
49. Cuiyun Hu, Caifang Yu, Yanhua Liu, Xianhui Hou, Xiaoyun Liu, Yunfei Hu* and Changwen Jin*. A Hybrid Mechanism for the Synechocystis Arsenate Reductase Revealed by Structural Snapshots during Arsenate Reduction. J Biol Chem. 290, 22262-22273, 2015
48. Yunfei Hu1, Yujie Wu1, Qianwen Li, Wenbo Zhang and Changwen Jin*. Solution Structure of Yeast Rpn9: Insights for Proteasome Lid Assembly. J. Biol. Chem. 290, 6878-6889, 2015 ( 1 equal contributions )
47. Qian Ye, Wenyu Fu, Yunfei Hu* and Changwen Jin* NMR structure note: Long-chain flavodoxin FldB from Escherichia coli. J. Biomol. NMR 60, 283-288, 2014
46. Qian Ye, Yunfei Hu* and Changwen Jin*. Conformational Dynamics of Escherichia coli Flavodoxins in Apo- and Holo-States by Solution NMR Spectroscopy. PLoS One. 9, e103936, 2014
45. Yi Zhang, Yunfei Hu*, Hongwei Li and Changwen Jin*. Structural Basis for TatA Oligomerization: An NMR Study of Escherichia coli TatA Dimeric Structure. PLoS One. 9, e103157, 2014
44. Yi Zhang, Lei Wang, Yunfei Hu* and Changwen Jin*. Solution structure of the TatB component of the twin-arginine translocation system. Biochim Biophys Acta. 1838, 1881-1888, 2014
43. Guoming Li, Linglong Qu, Shuaipeng Ma, Yujie Wu, Changwen Jin* and Xiaofeng Zheng*. Structure determination of human Fas apoptosis inhibitory molecule and identification of the critical residues linking the interdomain interaction to the anti-apoptotic activity. Acta Crystallogr D Biol Crystallogr. 70, 1812-1822, 2014
42. Yujie Wu, Yunfei Hu* and Changwen Jin*.1H, 13C and 15N resonance assignments of the VWA domain of Saccharomyces cerevisiae Rpn10, a regulatory subunit of 26S proteasome. Biomol. NMR Assign. 8, 391-394, 2014
41. Yujie Wu, Yunfei Hu* and Changwen Jin*.1H, 13C and 15N resonance assignments of Rpn9, a regulatory subunit of 26S proteasome from Saccharomyces cerevisiae. Biomol. NMR Assign. 8, 307-311, 2014
40. Qian Ye, Yunfei Hu* and Changwen Jin*.1H, 13C and 15N resonance assignments of the apo and holo states of flavodoxin YqcA from Escherichia coli. Biomol. NMR Assign. 8, 269-273, 2014
39. Tingting Liu, Zixu Liu, Chuanjun Song, Yunfei Hu, Zhifu Han, Ji She, Fangfang Fan, Jiawei Wang, Changwen Jin, Junbiao Chang, Jian-Min Zhou, Jijie Chai*. Chitin-induced dimerization activates a plant immune receptor. Science 336, 1160-1164, 2012
38. Yunfei Hu, Xinxin Zhang, Yunming Shi, Yanfeng Zhou, Wei Zhang, Xiao-Dong Su, Bin Xia, Jindong Zhao* and Changwen Jin*. Structures of Anabaena calcium-binding protein CcbP: Insights into Ca2+ signalling during heterocyst differentiation. J. Biol. Chem. 2011, doi:10.1074/jbc.M110.201186 [Supplemental Data]
37. Caifang Yu, Bin Xia, and Changwen Jin*. 1H, 13C and 15N resonance assignments of the arsenate reductase from Synechocystis sp. strain PCC 6803. Biomol. NMR Assign. 5, 85-87, 2011
36. Hongwei Li, Bin Xia, and Changwen Jin*. 1H, 13C and 15N resonance assignments of rhodanese GlpE from Escherichia coli. Biomol. NMR Assign. 5, 97-99, 2011
35. Hongwei Li, Yunchen Bi, Bin Xia, and Changwen Jin*. 1H, 13C and 15N resonance assignments of the rhodanese domain of YgaP from Escherichia coli. Biomol. NMR Assign. 5, 101-103, 2011
34. Jiang Hong1, Yunfei Hu1, Congmin Li, Zongchao Jia, Bin Xia and Changwen Jin*. NMR characterizations of the ice binding surface of an antifreeze protein. PLoS One. 5, e15682, 2010 ( 1 equal contributions )
33. Yunfei Hu, Enwei Zhao, Hongwei Li, Bin Xia, and Changwen Jin*. Solution NMR structure of the TatA component of the twin-arginine protein transport system from Gram-positive bacterium Bacillus sutilis. J. Am. Chem. Soc. 132, 15942-15944, 2010 [Supplemental Data]
32. Heng Peng, Yunfei Hu, Aiguo Zhou, Changwen Jin, and Weiqing Pan*. Solution structure of a Plasmodium falciparum AMA-1/MSP 1 chimeric protein vaccine candidate (PfCP-2.9) for malaria. Malar. J. 9, 76-84, 2010
31. Lingxiang Jiang, Caifang Yu, Manli Deng, Changwen Jin, Yilin Wang*, Yun Yan, and Jianbing Huang*. Selectivity and stoichiometry boosting of β-cyclodextrin in cationic/anionic surfactant systems: When host-guest equilibrium meets biased aggregation equilibrium. J. Phys. Chem. B. 114, 2165-2174, 2010
30. 胡蕴菲，金长文*. 蛋白质溶液结构及动力学的核磁共振研究（特邀综述）波谱学杂志 26(2), 151-172, 2009
29. Huanhuan Liang, Hao Chen, Keqiang Fan, Ping Wei, Xianrong Guo, Changwen Jin, Chen Zeng, Chao Tang, and Luhua Lai*. De novo design of a βαβ motif. Angew. Chem. Int. Ed. Engl. 48, 3301-3276, 2009
28. Jascha Blobel, Pau Bernado, Huimin Xu, Changwen Jin, and Miquel Pons*. Weak oligomerization of low-molecular-weight protein tyrosine phosphatase is conserved from mammals to bacteria. FEBS J. 276,4346-4357, 2009
27. Yunchen Bi, Hongwei Li, Shoujin Fan*, Bin Xia, and Changwen Jin*. 1H, 13C and 15N resonance assignments of RNA pyrophosphohydrolase RppH from Escherichia coli. Biomol. NMR Assign. 3,149-151, 2009
26. Xuan Shao, Jie Lu, Bin Xia, and Changwen Jin*. 1H, 13C and 15N resonance assignments of the chaperone HybE of hydrogenase-2 from Escherichia coli. Biomol. NMR Assign. 3,129-131, 2009
25. Jie Lu, Xu Wang, Bin Xia, and Changwen Jin*. Solution structure of apo-YjaB from Escherichia coli. PROTEINS: Structure, Function, and Bioinformatics 76, 261-265, 2009
24. Xuan Shao, Jie Lu, Yunfei Hu, Bin Xia, and Changwen Jin*. Solution structure of the Escherichia coli HybE reveals a novel fold. PROTEINS: Structure, Function, and Bioinformatics 75,1051-1056, 2009
23. Run-Chao Lu, Xianrong Guo, Changwen Jin, and Jin-Xin Xiao*. NMR studies on binding sites and aggregation-disassociation of fluorinated surfactant sodium perfluorooctanoate on protein ubiquitin. Biochim. Biophys. Acta. 1790, 134-140, 2009
22. Ewen Lescop, Zhenwei Lu, Qin Liu, Huimin Xu, Guangyu Li, Bin Xia, Honggao Yan* and Changwen Jin*. Dynamics of the conformational transitions in the assembling of the Michaelis complex of a bisubstrate enzyme: a 15N relaxation study of Escherichia coli 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase. Biochemistry 48, 302-312, 2009
21. Jie Lu, Fan Yang, You Li, Bin Xia, and Changwen Jin*. 1H, 13C, and 15N resonance assignments of the reduced and oxidized forms of Bacillus subtilis thiol peroxidase. Biomol. NMR Assign. 2, 183-186, 2008
20. Jie Lu, Fan Yang, You Li, Xinxin Zhang, Bin Xia, and Changwen Jin*. Reversible conformational switch revealed by the redox structures of Bacillus subtilis thiol peroxidase. Biochem. Biophys. Res. Commun. 373(3), 414-418, 2008
19. Hongwei Li, Fan Yang, Xue Kang, Bin Xia, and Changwen Jin*. Solution structures and backbone dynamics of Escherichia coli rhodanese PspE in its sulfur-free and persulfide-intermediate forms: implications for the catalytic mechanism of rhodanese. Biochemistry 47, 4377-4385, 2008
18. Caifang Yu, You Li, Bin Xia, and Changwen Jin*. Solution structure of the cryptic mannitol-specific phosphotransferase enzyme IIA CmtB from Escherichia coli. Biochem. Biophys. Res. Commun. 362(4), 1001-1006, 2007
17. Wenyu Fu, Fan Yang, Xue Kang, Xinxin Zhang, You Li, Bin Xia, and Changwen Jin*. First structure of the polymyxin resistance proteins. Biochem. Biophys. Res. Commun. 361(4), 1033-1037, 2007
16. Lei Wang, Bin Xia, and Changwen Jin*. Solution structure of Escherichia coli HypC. Biochem. Biophys. Res. Commun. 361(3), 665-669, 2007
15. You Li, Yunfei Hu, Wenyu Fu, Bin Xia, and Changwen Jin*. Solution structure of the bacterial chemotaxis adaptor protein CheW from Escherichia coli. Biochem. Biophys. Res. Commun. 360(4), 863-867, 2007
14. Fei Wang, Fan Yang, Yunfei Hu, Xu Wang, Xinhe Wang, Changwen Jin, and Jiyan Ma*. Lipid interaction converts prion protein to a PrPSc-like proteinase K-resistant conformation under physiological conditions. Biochemistry 46(23), 7045-7053, 2007 [Supplemental Data]
13. You Li1, Yunfei Hu1, Xinxin Zhang, Huimin Xu, Ewen Lescop, Bin Xia, and Changwen Jin*. Conformational fluctuations coupled to the thiol-disulfide transfer between thioredoxin and arsenate reductase in Bacillus subtilis. J. Biol. Chem. 282(15), 11078-11083, 2007 ( 1 equal contributions ) [Supplemental Data]
12. Fan Yang, Wei Hu, Huimin Xu, Congmin Li, Bin Xia, and Changwen Jin*. Solution structure and backbone dynamics of an endopeptidase HycI from Escherichia coli: implications for mechanism of the [NiFe] hydrogenase maturation. J. Biol. Chem. 282(6), 3856-3863, 2007
11. Yunfei Hu, You Li, Xinxin Zhang, Xianrong Guo, Bin Xia, and Changwen Jin*. Solution structures and backbone dynamics of a flavodoxin MioC from Escherichia coli in both apo- and holo-forms: implications for cofactor binding and electron transfer. J. Biol. Chem. 281(46), 35454-35466, 2006 [Supplemental Data]
10. Ewen Lescop, Yunfei Hu, Huimin Xu, Wei Hu, Juan Chen, Bin Xia, and Changwen Jin*. The solution structure of Escherichia coli Wzb reveals a novel substrate recognition mechanism of prokaryotic low molecular weight protein tyrosin phophatases. J. Biol. Chem. 281(28), 19570-19577, 2006 [Supplemental Data]
9. Xue Kang, Yunfei Hu, You Li, Xianrong Guo, Xiaolu Jiang, Luhua Lai, Bin Xia, and Changwen Jin*. Structural, biochemical and dynamic characterizations of the hRPB8 subunit of human RNA polymerases. J. Biol. Chem. 281(26), 18216-18226, 2006 [Supplemental Data]
8. Xinxin Zhang, Yunfei Hu, Xianrong Guo, Ewen Lescop, You Li, Bin Xia, and Changwen Jin*. The Bacillus subtilis YKUV is a thiol-disulfide oxidoreductase revealed by its redox structures and activity. J. Biol. Chem. 281(12), 8296-8304, 2006
7. Huimin Xu, Bin Xia, and Changwen Jin*. Solution structure of a low-molecular-weight protein tyrosine phosphatase from Bacillus subtilis. J. Bacteriol. 188(4), 1509-1517, 2006
6. Xianrong Guo1, You Li1, Kuan Peng, Yunfei Hu, Congmin Li, Bin Xia, and Changwen Jin*. Solution structures and backbone dynamics of arsenate reductase from Bacillus subtilis: reversible conformational switch associated with arsenate reduction. J. Biol. Chem. 280(47), 39601-39608, 2005 ( 1 equal contributions )
5. Xinxin Zhang, Caifang Yu, Bin Xia, and Changwen Jin*. NMR assignment of new thioredoxin-like protein YkuV from Bacillus subtilis. J. Biomol. NMR 32, 258, 2005
4. Congmin Li, Xianrong Guo, Zongchao Jia, Bin Xia, and Changwen Jin*. Solution structure of an antifreeze protein CfAFP-501 from Choristoneura fumiferana. J. Biomol. NMR 32, 251-256, 2005
3. Huimin Xu, Peng Zhang, and Changwen Jin*. Letter to the Editor. NMR assignments of a low molecular weight protein tyrosine phosphatase (PTPase) from Bacillus subtilis. J. Biomol. NMR 31, 363, 2005
2. Congmin Li, and Changwen Jin*. Letter to the Editor: 1H, 13C and 15N resonance assignments of the antifreeze protein cfAFP-501 from spruce budworm at different temperatures. J. Biomol. NMR 30, 101-102, 2004
1. Kuan Peng, Xiaodong Su, Bin Xia, and Changwen Jin*. Letter to the Editor: 1H, 13C and 15N resonance assignments of a Bacillus subtilis arsenate reductase. J. Biomol. NMR 29, 459－460, 2004
